MIrROR (Microbial Identification using rRNA Operon Region) is a platform for metataxonomics using the 16S-23S rRNA operon sequence. MIrROR provides a curated database for rRNA operon and analysis tools, facilitating high-resolution microbial identification and research. Our latest release incorporates significant improvements and expanded functionalities to support cutting-edge microbial research.
| Collected microbial genomes | Total operon sequence |
| 1,690,470 | 476,579 |
| Genomes covering operons | Species covering operons |
| 249,907 | 29,051 |
MIrROR v.2.0 has collected 3.6 times more GenBank bacterial genomes compared to the previous version, and all genomes have been reclassified according to GTDB Release 220 (updated on April 24, 2024). This reflects the latest taxonomy to ensure accurate and up-to-date microbial identification and classification.
| Phylum | Class | Order | Family | Genus | Species | |
| Bacteria | 137 | 327 | 890 | 1,897 | 6,925 | 27,718 |
| Archaea | 19 | 44 | 85 | 243 | 559 | 1,333 |
The extracted operon sequences underwent initial curation, which included removing duplicate sequences (within-genome and inter-species), sequences with ambiguous nucleotides, and genomes assigned unclear taxonomy. Subsequently, to remove contaminated sequences generated due to low assembly quality, sequencing errors, etc., >99% identity clustering and curation was performed.
Seol, Donghyeok, et al. (2022) “Microbial Identification Using rRNA Operon Region: Database and Tool for Metataxonomics with Long-Read Sequence.” Microbiology Spectrum.
https://doi.org/10.1128/spectrum.02017-21